Part:BBa_K4711002
NeuAc riboswitch+ phiX174
In order to prevent E. coli from leaking into the environment, we designed the NeuAc ribose switch. The sequence of the NeuAc riboswitch, which consists of an RNA aptamer and a hammerhead ribozyme. The principle of its action is that NeuAc binding to the RNA aptamer causes the conformational change of the entire switch, resulting in hammerhead ribozyme self-shearing, resulting in a gap, and then under the action of ReJ ribozyme in Escherichia coli cells, the subsequent genes are clipped, thereby reducing the abundance of mRNA of downstream genes [16-17]. In order to ensure that the switch can work effectively, there are spacers at both ends of the switch, and the 5' end is 5-AAAAATAAAAAGAAAA-3, and the 3 'end is 5-AAAAAACAAAA-3. We selected ΦX174 as a lytic protein downstream to achieve E. coli suicide.
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
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